OPTIMIZATION OF DNA EXTRACTION PROTOCOLS FROM SHRIMP (Penaeus monodon) TISSUE

DNA analyses and DNA-based diagnoses are going forwardly in the commercial deals with shrimp (Penaeus monodon) as well basic research. In this regard extraction of is obvious. However, numerous protocols available from animal tissue. Therefore, which technique suitable one for tissue indispensable to justify. current study, a protocol was optimized collected Batiaghata, Khulna. Muscle Tissue (MT) Hepatopancreas (HT) were distinctly sampled on15th March, 2007 only fresh individual each six applied. Guanidium isothiocyanate treatment involving two groups preparation (DNAzol invitrogen® Extraction Buffer (DEB) Genei®) or without RNAse, Proteinase K isolation sodium dodecyle sulfate (SDS) applied extract DNA. The quantity (µgml-1) quality determined by UV spectrophotometry at A260nm A260/280 respectively. study provided that obtained almost every higher when hepatopancreas used. Among protocols, DEB RNAse (102.5 µgml-1 MT, 302.5 HT) resulted highest concentration acceptable level purity (A260/280 1.86 MT 1.70 HT).